The starting team contains five players who'll be wearing the famous red jersey for the first time, while a further six men on the replacements bench have yet to represent the Lions.
Six of the bench-sitters last Saturday, however, have been elevated to the starting XV.
"We were always going to mix it up in the first three matches so that everyone gets a full outing," said Lions coach Ian McGeechan on Monday when the team was announced.
"The first three matches of this tour were set aside so that we could have a look at everyone."
"They are all very experienced internationals and previous Lions tourists," said McGeechan.
"David Wallace now has the opportunity to play at openside flanker following his selection at No 8 against the Royal XV.
"Stephen Jones who was a replacement in the last match, but did not get a run, links up with his Welsh team-mate and scrumhalf Mike Phillips giving the team an experienced halfback combination."
McGeechan added that the team had done a lot work on several key areas since Saturday's match in Rustenburg.
"We're just one game into the tour, but have to keep developing our game. Hopefully we'll take a few more steps in the right direction on Wednesday."
Meanwhile, the Golden Lions team, also named on Monday morning, shows no significant changes to the side that featured during this year's Super 14 competition.
British and Irish Lions team (15-1)
Rob Kearney; Tommy Bowe, Brian O'Driscoll, Jamie Roberts, Ugo Monye; Stephen Jones, Mike Phillips; Jamie Heaslip, David Wallace, Tom Croft, Alun-Wyn Jones, Nathan Hines, Phil Vickery, Lee Mears, Gethin Jenkins
Golden Lions team (15-1)
Louis Ludik; Michael Killian, Jannie Boshoff, Doppies la Grange, Dusty Noble; Andre Pretorius, Jano Vermaak; Willem Alberts, Franco van der Merwe, Cobus Grobbelaar (capt); Willem Stoltz, Brad Mockford, Gert Muller, Willie Wepener, Lawrence Sephaka. Replacements: Ethienne Reynecke, JC Janse van Rensburg, Ernst Joubert, Todd Clever, Chris Jonck, Walter Venter, Earl Rose
Source: AFP European Edition